An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.
Identifieur interne : 000A58 ( Main/Exploration ); précédent : 000A57; suivant : 000A59An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.
Auteurs : L. Hildén [Suède] ; L. Eng ; G. Johansson ; S E Lindqvist ; G. PetterssonSource :
- Analytical biochemistry [ 0003-2697 ] ; 2001.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Cellulase.
- composition chimique : Carbohydrate dehydrogenases.
- enzymologie : Phanerochaete.
- méthodes : Techniques de biocapteur.
- Contrôle de qualité, Facteurs temps, Reproductibilité des résultats, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Cellulase.
- chemical , chemistry : Carbohydrate Dehydrogenases.
- enzymology : Phanerochaete.
- methods : Biosensing Techniques.
- Evaluation Studies as Topic, Quality Control, Reproducibility of Results, Time Factors.
Abstract
The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.
DOI: 10.1006/abio.2000.4959
PubMed: 11237326
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Eng</name></author><author><name sortKey="Johansson, G" sort="Johansson, G" uniqKey="Johansson G" first="G" last="Johansson">G. Johansson</name></author><author><name sortKey="Lindqvist, S E" sort="Lindqvist, S E" uniqKey="Lindqvist S" first="S E" last="Lindqvist">S E Lindqvist</name></author><author><name sortKey="Pettersson, G" sort="Pettersson, G" uniqKey="Pettersson G" first="G" last="Pettersson">G. 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Hildén</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, Uppsala University, SE-751 23 Uppsala, Sweden. lars.hilden@biokem.uu.se</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Biochemistry, Uppsala University, SE-751 23 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Eng, L" sort="Eng, L" uniqKey="Eng L" first="L" last="Eng">L. Eng</name></author><author><name sortKey="Johansson, G" sort="Johansson, G" uniqKey="Johansson G" first="G" last="Johansson">G. Johansson</name></author><author><name sortKey="Lindqvist, S E" sort="Lindqvist, S E" uniqKey="Lindqvist S" first="S E" last="Lindqvist">S E Lindqvist</name></author><author><name sortKey="Pettersson, G" sort="Pettersson, G" uniqKey="Pettersson G" first="G" last="Pettersson">G. Pettersson</name></author></analytic><series><title level="j">Analytical biochemistry</title><idno type="ISSN">0003-2697</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Carbohydrate Dehydrogenases (chemistry)</term><term>Cellulase (analysis)</term><term>Evaluation Studies as Topic (MeSH)</term><term>Phanerochaete (enzymology)</term><term>Quality Control (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Carbohydrate dehydrogenases (composition chimique)</term><term>Cellulase (analyse)</term><term>Contrôle de qualité (MeSH)</term><term>Facteurs temps (MeSH)</term><term>Phanerochaete (enzymologie)</term><term>Reproductibilité des résultats (MeSH)</term><term>Techniques de biocapteur (méthodes)</term><term>Études d'évaluation comme sujet (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cellulase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carbohydrate Dehydrogenases</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Cellulase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Carbohydrate dehydrogenases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques de biocapteur</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Evaluation Studies as Topic</term><term>Quality Control</term><term>Reproducibility of Results</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Contrôle de qualité</term><term>Facteurs temps</term><term>Reproductibilité des résultats</term><term>Études d'évaluation comme sujet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11237326</PMID><DateCompleted><Year>2001</Year><Month>05</Month><Day>03</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-2697</ISSN><JournalIssue CitedMedium="Print"><Volume>290</Volume><Issue>2</Issue><PubDate><Year>2001</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal Biochem</ISOAbbreviation></Journal><ArticleTitle>An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.</ArticleTitle><Pagination><MedlinePgn>245-50</MedlinePgn></Pagination><Abstract><AbstractText>The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.</AbstractText><CopyrightInformation>Copyright 2001 Academic Press.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hildén</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Uppsala University, SE-751 23 Uppsala, Sweden. lars.hilden@biokem.uu.se</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eng</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Johansson</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Lindqvist</LastName><ForeName>S E</ForeName><Initials>SE</Initials></Author><Author ValidYN="Y"><LastName>Pettersson</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Biochem</MedlineTA><NlmUniqueID>0370535</NlmUniqueID><ISSNLinking>0003-2697</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 1.1.-</RegistryNumber><NameOfSubstance UI="D002237">Carbohydrate Dehydrogenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.99.18</RegistryNumber><NameOfSubstance UI="C019859">cellobiose-quinone oxidoreductase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002237" MajorTopicYN="N">Carbohydrate Dehydrogenases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005069" MajorTopicYN="N">Evaluation Studies as Topic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011786" MajorTopicYN="N">Quality Control</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>3</Month><Day>10</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>5</Month><Day>5</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>3</Month><Day>10</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11237326</ArticleId><ArticleId IdType="doi">10.1006/abio.2000.4959</ArticleId><ArticleId IdType="pii">S0003-2697(00)94959-5</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country><region><li>East Middle Sweden</li><li>Svealand</li></region><settlement><li>Uppsala</li></settlement><orgName><li>Université d'Uppsala</li></orgName></list><tree><noCountry><name sortKey="Eng, L" sort="Eng, L" uniqKey="Eng L" first="L" last="Eng">L. Eng</name><name sortKey="Johansson, G" sort="Johansson, G" uniqKey="Johansson G" first="G" last="Johansson">G. Johansson</name><name sortKey="Lindqvist, S E" sort="Lindqvist, S E" uniqKey="Lindqvist S" first="S E" last="Lindqvist">S E Lindqvist</name><name sortKey="Pettersson, G" sort="Pettersson, G" uniqKey="Pettersson G" first="G" last="Pettersson">G. Pettersson</name></noCountry><country name="Suède"><region name="Svealand"><name sortKey="Hilden, L" sort="Hilden, L" uniqKey="Hilden L" first="L" last="Hildén">L. Hildén</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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